Hoàn thiện công nghệ sản xuất nấm men đối kháng kết hợp với màng bao ăn được để bảo quản quả thanh long

1






; Khoa Sinh hc
 ; 60 42 40
h:GS-



Abstract. 














: , 







 



 . 
 .

: 

 
 . 































. 









 




 27 
8-

Keywords. ; ; 


Content:

thanh long 
Candida sake     
men Candida sake A.niger 
Colletotrichum gloeosporioides thanh long. 
“Hon thin công ngh sản xuất nấ m men đối kháng kết hợp
với mng bao ăn được để bảo quản thanh long”

2
MỤC TIÊU CỦA ĐỀ TÀI
- P

  Candida sake 
 Aspergillus niger  Colletotrichum
gloeosporioides  thanh long.
- Candida
sake thanh long 

o
C
NỘI DUNG NGHIÊN CỨU
- 



  Candida sake 








: , 







   

.
- 


thanh long.
-  









 thanh
long Aspergillus niger  Colletotrichum gloeosporioides 
men Candida sake 
- 















 
Candida sake .
- 











 Candida sake 
 27  h 8-
10
0
C.
CHƢƠNG I: TỔNG QUAN






 
             Asperigllus,
Cladosporium, Botritis CinereaAltenaria, Aspergillus,
Cladosporium, Colletotrichum, PhomopsisFusarium 
  Penicillium, Phoma, Phytophthora, Pithyum  Rhizopus   
   Ceratocystis fimbriata, Rhizoctonia solani, Sclerotoni sclerotonum,
gleosporoinos. 





3



Candida sake Candida 
Candida sake 
Candida sake 


.
Tnh hnh sản xut v bảo quản thanh long  Vit Nam
 Hylocereus undatus Hylocereus,
 Hylocereus polyrhizus Hylocereus
     Hylocereus megalanthus       
Selenicereus thanh long 






 


,85kg/cm


an















thanh long
 Aspergillus niger, Aspergillus flavus , Penicillium,
Colletotrichum gloeosporioides thanh long.
  Aspergillus niger 





 , 
Thanh long.
  Colletotrichum gloeosporioides  





 thanh
long
CHƢƠNG 2 : VẬT LIỆU VÀ PHƢƠNG PHÁP NGHIÊN CỨU

4
2.1 Vật liu nghiên cứu
2.2 Phƣơng pháp nghiên cứu
thanh long

thanh
long
 Candida sake   
thanh long
Candida sake 
 
 





 Candida sake 







 Candida sake 

thanh long 









 



thanh long Candida
sake 

Phcel
CHƢƠNG 3:KẾT QUẢ VÀ THẢO LUẬN
3.1. Kết quả phân lập chủng nm men đối kháng

Nguồn phân lập
Số
mẫu
Số chủng nm men
đối kháng phân lập
Tỷ l chủng nm
men đối kháng / số

5
đƣợc
mẫu thu thập (%)

(
120
32
26,7

()
98
23
23,5

218
55
25,2


thanh long 
:
  A.niger  Thanh long   
.
  
Candida 

A.niger  
    

    

    

55
40
13
2

6
 cA.niger
sau 7  A.niger 

A.niger 
      Colletotrichum gloeosporioides    Thanh
long 
  
Candida  

Colletotrichum gloeosporioides 

   

    



55
40
14
1

            
Colletotrihcum gloeosporioides A.niger 
Colletotrichum gloeosporioides A.niger 

7
.
Colletotrichum gloeosporioides 




L1.5.4 
-  





Urease
Glucose

Maltose
Sucrose
Lactose

8
Candida
sake L1
5.4
+
+
+
-
+

3.2. Định loại chủng nm men đối kháng
2,5s  

ID CsVn_ITS PRELIMINARY; DNA; 708 BP.
SQ SEQUENCE 708 BP; 212 A; 139 C; 156 G; 201 T;
TCCTGGTCAT TTAGAGGAAG TAAAAGTCGT AACAAGGTTT
CCGTAGGTGA CCCTGCGGAA 60
GGATCATTAA AGTTTGCTTA ATTGCATTAC ACATGTTTTT
TTAGAGAACT TGCTTGCAAG 120
AACACTAATA ATTTACTTAG TCAACAAATA AAAATATCAA
AACTTTCAAC AACGGATCTC 180
TTGGTTCTCG CATCGATGAA GAACGCAGCG AAATGCGATA
CGTAATATGA ATTGCAGATT 240
TTCGTGAATC TTCGAATCTT TGAACGCACA TTGCGCCCTG
TGGTATTCCA CAGGGCATGC 300
CTGTTTGAGC GTCATTTCTC CCTCAAACCT CTGGTTTGGC
GTTGAGTGAT ACTCGGTTTA 360
CTTGAAAAAC ATGAAAAGCA TAACTATTAG GTTTTACCAA
CTCGTTATAC TAATCTACAA 420
GTTTGACCTC AAATCAGGTA GGACTAACCG CTGAACTTAA
GCATATCAAT TAGCGGAGGA 480
AAAGAAACCA ACCGGGATTG GCTCAGTAAC GGCGAGTGAA
GCGGCAAAAG CTCAAATTTG 540

9
AAATCTGCGT AAGCCGAGTT GTAATTTGAA GATGGCTACT
TTGGTAATGG CTCTTGTCTA 600
TGTTCCTTGG AACAGGACGT CACAGAGGGT GAGAATCCCG
TGCGATGAGA TGTCCATTAC 660
CGTGTAAAGT GCTTTCGAAG AGTCGAGTTG TTTGCCAATG
CAGCTCTA 708
3.3 Đánh giá hiu quả bảo quản của các công thức mng bao
3.3.1 Candida sake L1.5.4 

Candida sake L1.5.4 
bao 

Candida sake L1.5.4(CFU/ml)




1,81.10
8

2,12.10
7

6,1.10
6


2,19.10
8

7,3.10
7

1,23.10
7


2,86.10
8

1,05.10
8

0,59.10
8

3thanh long 
Thanh long 
















(mgCO2/k
g/h)





30
6,15
11,9
32,4





Chitosan

30

3.48

6,25

18,6










10

CT27
30
3,15
5,2
21.5






CT27

30

2,29

5,15

20,9






Kết luận: 
               
thanh long 
3.4 Kết quả khảo sát yếu tố nhân nuôi thích hợp chủng nm men Candida sake
L1.5.4




PH




Malt
6,5
30oC
100%

3.5. Kết quả thử nghim bảo quản quả thanh long bằng chế phẩm Candida sake
L1.5.4 đối kháng kết hợp với mng bao ăn đƣợc CT27 cải tiến  quy mô phòng
thí nghim
thanh long  28
0

Candida sake L1.5.4 
Chỉ tiêu
Lô đối chứng
Lô thí nghim





97 - 98
7 - 8










11




96 - 97
6 - 7
thanh long m men Candida sake L1.5.4
- 10
0
C
Chỉ tiêu
Lô đối chứng
Lô thí nghim



    

65 - 66
1- 1,5



 









65 - 66
0,5 - 1
3.6. Kết quả xây dựng mô hnh bảo quản quả thanh long bằng chế phẩm nm
men đối kháng Candida sake kết hợp với mng bao ăn đƣợc tại Bnh Thuận
3.6.1   
 thanh long 
:
: 
: 
 :         

:

:  

: 

12


3.6.2 
 :

o


. y

 

3.6.3  Lan Anh








:

Candida sake  
thanh long 


CHƢƠNG 4: KẾT LUẬN

 Candida sake ng
Colletotrichum gloeosporioides A.niger

13
  












Candida sake L1.5.4 





 : 


, pH=6,5; 



30
o
C, 



100%.
  

 L

            Candida sake
 
8  10
o
C  

References :
1. Tiê
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ng viê
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[1]: 

  













 : 

 2007-
2009
[2]: 

:  01/2007 



















[3]: 
2

2


-66
[4]:               

http://www.nacentech.ac.vn
[5]: 

-CN--2002

14
[6]:  
Thanh long 
-2002, Vi-387.
[7]: ,
          

- - 377
[8]: (2004) thanh 

:
[9]:  C

2. Tiê
́
ng anh
[10]: Hardenburg, 1967; Baldwin 1994
[11]: 





, 1999
[12]: Kaplan, 1986
[13]: Baldwin E. A., Baker R. A.(2002): Use of protein in edible coatings for whole
and minimally processed fruits and vegetales. In: Protein-based Films and Coatings.
Gennadios A. (Ed.). CRC Press, Boca Raton. FL. 501-515.
[14]: Plotto A., Baker B., 2005: Review of wax-based and other coatings for fruits
and vegetables. IFOAM Technical Paper, July 14, 2005
[15]: Fusarium
p. 364-369
[16]: google.com-rauhoaquavietnam.vn
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strategy for microbial antagonists to control postharvest diseases of fruits and
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